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cell signalling technology 9860s  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cell signalling technology 9860s
    Cell Signalling Technology 9860s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 3674 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/senescent+cells/Senescence+beta-Galactosidase+Staining+Kit/pmc13018883-55-14-14
    Average 97 stars, based on 3674 article reviews
    cell signalling technology 9860s - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Chemical Composition and Effect against Skin Alterations of Bioactive Extracts Obtained by the Hydrodistillation of Eucalyptus globulus Leaves
    Article Snippet: HaCaT and NIH/3T3 cells were seeded in 12-well plates at a density of 1.5 × 10 5 or 2.5 × 10 4 cells/well, respectively, and were allowed to adhere for 24 h. Thereafter, cellular senescence was induced with 100 μM etoposide (Sigma-Aldrich, St. Louis, MO, USA) for 72 h or 12.5 μM etoposide for 24 h in the HaCaT and NIH/3T3 cells, respectively. .. After the incubation period, the senescent cells and controls (exposed to etoposide-free medium) were treated in the absence or presence of 0.16 mg/mL EO or 0.8 μg/mL HRW for 24 h. Then, the culture medium was discarded, the cells were washed with PBS, fixed, and stained with freshly prepared β-galactosidase (β-gal) staining solution following the protocol provided by the manufacturer (Cell Signaling Technology, Danvers, MA, USA). .. Finally, the senescent cells were quantified using a widefield microscope (Carl Zeiss, Oberkochen, Germany) at a magnification of 40× by determining the percentage of β-gal-positive cells in randomly selected fields of four microscopic images, in at least three independent experiments performed in duplicate.

    Staining:

    Article Title: Chemical Composition and Effect against Skin Alterations of Bioactive Extracts Obtained by the Hydrodistillation of Eucalyptus globulus Leaves
    Article Snippet: HaCaT and NIH/3T3 cells were seeded in 12-well plates at a density of 1.5 × 10 5 or 2.5 × 10 4 cells/well, respectively, and were allowed to adhere for 24 h. Thereafter, cellular senescence was induced with 100 μM etoposide (Sigma-Aldrich, St. Louis, MO, USA) for 72 h or 12.5 μM etoposide for 24 h in the HaCaT and NIH/3T3 cells, respectively. .. After the incubation period, the senescent cells and controls (exposed to etoposide-free medium) were treated in the absence or presence of 0.16 mg/mL EO or 0.8 μg/mL HRW for 24 h. Then, the culture medium was discarded, the cells were washed with PBS, fixed, and stained with freshly prepared β-galactosidase (β-gal) staining solution following the protocol provided by the manufacturer (Cell Signaling Technology, Danvers, MA, USA). .. Finally, the senescent cells were quantified using a widefield microscope (Carl Zeiss, Oberkochen, Germany) at a magnification of 40× by determining the percentage of β-gal-positive cells in randomly selected fields of four microscopic images, in at least three independent experiments performed in duplicate.

    Article Title: Biglycan Alleviates Age-Related Muscle Atrophy and Hepatocellular Senescence.
    Article Snippet: .. After treatment, the cells were rinsed with PBS and stained to identify senescent cells using a commercial kit (Cell Signaling Technology, Danvers, MA, USA) in accordance with the manufacturer’s protocol. .. Following staining, the cells were examined using a light microscope, and the β-galactosidase-positive areas were quantified with ImageJ software (NIH, Bethesda, MD, USA).

    Activity Assay:

    Article Title: β-Asarone induces senescence in colorectal cancer cells by inducing lamin B1 expression.
    Article Snippet: Colorectal cancer is a leading cause of cancer mortality with a complex carcinogenesis that includes reduced cellular senescence.. Lamin proteins are decreased in senescing cells, and frequently decreased in malignancies.. This study identified a new drug candidate for colorectal cancer that appears to target cell senescence via a lamin protein.

    Positive Control:

    Article Title: β-Asarone induces senescence in colorectal cancer cells by inducing lamin B1 expression.
    Article Snippet: Colorectal cancer is a leading cause of cancer mortality with a complex carcinogenesis that includes reduced cellular senescence.. Lamin proteins are decreased in senescing cells, and frequently decreased in malignancies.. This study identified a new drug candidate for colorectal cancer that appears to target cell senescence via a lamin protein.

    Chromatin Immunoprecipitation:

    Article Title: β-Asarone induces senescence in colorectal cancer cells by inducing lamin B1 expression.
    Article Snippet: Colorectal cancer is a leading cause of cancer mortality with a complex carcinogenesis that includes reduced cellular senescence.. Lamin proteins are decreased in senescing cells, and frequently decreased in malignancies.. This study identified a new drug candidate for colorectal cancer that appears to target cell senescence via a lamin protein.

    Western Blot:

    Article Title: β-Asarone induces senescence in colorectal cancer cells by inducing lamin B1 expression.
    Article Snippet: Colorectal cancer is a leading cause of cancer mortality with a complex carcinogenesis that includes reduced cellular senescence.. Lamin proteins are decreased in senescing cells, and frequently decreased in malignancies.. This study identified a new drug candidate for colorectal cancer that appears to target cell senescence via a lamin protein.



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    Image Search Results


    ISO induces senescence in the apex of the young adult rat. 10–12-week-old rats were administered 100 mg/kg ISO or saline for five days, and then apexes were collected on days 10 and 28. ( A ) Representative SA-β-gal staining images (blue; black arrows). ( B ) Quantification of SA-β-gal-positive area. ( C ) Representative p16 staining images (green; yellow arrows). ( D ) Quantification of p16-positive cells. ( E ) Representative p21 staining images (green; yellow arrows). ( F ) Quantification of p21-positive cells. Scale bars = 100 µm. Data are expressed as the mean ± S.D. Statistical analysis was performed using two-way ANOVA followed by Šídák’s multiple comparisons test. n = 5 per group.

    Journal: Biomedicines

    Article Title: Isoproterenol Induces Cardiac Injury and Senescence in Sprague–Dawley Rats: A Cost-Effective Pharmacological Model

    doi: 10.3390/biomedicines14071445

    Figure Lengend Snippet: ISO induces senescence in the apex of the young adult rat. 10–12-week-old rats were administered 100 mg/kg ISO or saline for five days, and then apexes were collected on days 10 and 28. ( A ) Representative SA-β-gal staining images (blue; black arrows). ( B ) Quantification of SA-β-gal-positive area. ( C ) Representative p16 staining images (green; yellow arrows). ( D ) Quantification of p16-positive cells. ( E ) Representative p21 staining images (green; yellow arrows). ( F ) Quantification of p21-positive cells. Scale bars = 100 µm. Data are expressed as the mean ± S.D. Statistical analysis was performed using two-way ANOVA followed by Šídák’s multiple comparisons test. n = 5 per group.

    Article Snippet: Frozen sections were used for SA-β-gal (HY-K1089, MedChemExpress, Monmouth Junction, NJ, USA) and p16 staining.

    Techniques: Saline, Staining